E3889

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E3889

Sigma

 

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

for molecular biology, ≥97.0%

Synonym:Chel™-DE, EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid
CAS Number:67-42-5
Linear Formula:[-CH2OCH2CH2N(CH2CO2H)2]2
Molecular Weight:380.35
Beilstein Registry Number:1717370
EC Number:200-651-2
MDL number:MFCD00004291
PubChem Substance ID:24894530

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Description

ApplicationA chelating agent useful for the determination of calcium in the presence of magnesium.
Legal InformationChel is a trademark of Ciba-Geigy AG

Properties

gradefor molecular biology
assay≥97.0%
loss≤1.0% loss on drying
EQP levelElite
mp241 °C (dec.)(lit.)
foreign activityDNase, RNase, and protease, none detected

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany2
RTECSAH3760000

References

referenceBrauner, P., Fridlender B., Use of chelating agents as terminators of alkaline phosphatase activity in enzyme-linked immunosorbent assay (ELISA) tests. J. Immunol. Methods 42, 375, (1981)
 Schmid, R.W., Reilly, C.N. Anal. Chem. 29, 264, (1957)
 Riley, W.W., Pfeiffer, D.R., Rapid and extensive release of Ca2+ from energized mitochondria induced by EGTA. J. Biol. Chem. 261, 28, (1986)
 Birch-Machin, M.A., Dawson, A.P., Effects of chelating agents on the Ca2+-stimulated ATPase of rat liver plasma membranes. Biochim. Biophys. Acta 855, 277, (1986)
 Vidal, H., et al., A test to evaluate the effect of individual components of ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid buffers on enzymatic activity. Anal. Biochem. 193, 135, (1991)
MerckMerck 13,3560
BeilsteinBeil. 4,IV,2433
 Corp MSDS 1, 1609:C / FT-IR 1 (1), 568:C / FT-IR 2 (1), 905:D / NMR-Reference 2 (1), 485:B / RegBook 1 (1), 651:B / Sax 6, 1339 / Sigma FT-IR 1 (2), 552:A / Structure Index 1, 95:C:4